α-细辛脑脂质聚合物杂化纳米粒的制备及表征
蒋楠;穆珺;王春柳;张红;刘洋;马虎强;李晔
【期刊名称】《陕西中医》
【年(卷),期】2018(039)006
【摘要】目的:探讨α-细辛脑脂质聚合物杂化纳米粒(ARE-LPHNPs)的工艺优化及表征.方法:采用自组装法制备α-细辛脑脂质聚合物杂化纳米粒,单因素试验考察卵磷脂与mPEG-PLGA的比例、mPEG-PLGA质量、有机相与水相体积比及药载比的影响,星点设计-效应面法优化处方工艺,并对其理化性质进行表征.结果:ARE-LPHNPs最优处方为mPEG-PLGA质量为4.69 mg、有机相与水相体积比1:2.08,药载比为19.74％.最优工艺制备出的ARE-LPHNPs外观呈球形,平均粒径及电位分别为(87.67±1.25)nm,(-18.1±0.99)mV,包封率与载药量分别为(84.17±0.37)％、(7.63±0.05)％.DSC结果表明药物在ARE-LPHNPs中未以结晶形式存在.结论:采用自组装法制备ARE-LPHNPs,工艺稳定,所制备纳米粒外观圆整,可以为进一步体内研究提供参考.%Objective:To optimize the formulation of the lipid polymer hybrid nanoparticles loaded with α-Asa-rone ,and characterize its physical and chemical properties .Methods:ARE-LPHNPs was prepared by using self-as-sembly method ,four factors including the ratio of lecithin to mPEG-PLGA ,the mass of the mPEG-PLGA ,volume ratio of organic phase to aqueous phase and the ratio of drug to mPEG-PLGA were investigated ,then to optimize the preparation of ARE-LPHNPs by the central composite design response surface method ,and to study its physico-chemical