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英文稿翻译
英文稿翻译

摘要。本研究开发的手持设备和低成本的数字全息显微镜(DHM)采用直列式全息图,一个摄像头,一个高功率RGB发光二极管(LED),和一个针孔。它的成本不到20000日元(约250美元在80日元/美元),约为120毫米和80毫米55毫米××在尺寸。此外,通过调整一个全息图的记录距离,横向在最合适的距离分辨率为17.5μM.此外,这种分布式水文模开发中使用的开放源代码库,因此是低成本和可容易被任何人了。在这项研究中,它的功能是降低成本和尺寸以提高横向分辨率进一步比现有的报告。这DHM将在现场,一个有用的应用教育,等等。关键词DHM,手持设备,成本低,网络摄像头,高功率RGB LED

手持设备和低成本的数字全息显微镜

简介

显微镜技术被应用在各种领域:(1)由于其高分辨率,(2)广泛的领域来看,和(3)的三维观测。光学显微镜的分辨率可以提高使用高透镜放大。然而,视野变窄。这在理论上是困难的用光学显微镜解决这个问题。数字全息显微术(DHM)试图利用光学原理不同,解决这个问题显微镜[ 1,2 ]。介绍了全息技术在显微镜技术原理,提供一个高分辨率和广泛的视野,例如参考文献[ 3 ]。此外,或使物体三维的观察。虽然DHM的光学系统是大型和昂贵的,低成本的分布式水文模型系统已报道的[ 4,5 ]。这两项研究使用的是数码相机的消费者为一个全息图记录装置。文献[ 4 ],建立了光学系统数码相机由佳能,激光二极管,和一些镜头。成本约1000美元,实现了横向2.8μM.系统分辨在光轴方向尺寸约35厘米。文献[ 4 ]是第一个提出了用数码相机为消费者提供低成本的分布式流域水文模型。然而,该系统不利于手持大小,和成本略高。此外,实时重建是困难的因为系统采用数码相机消费者在我们的研究中,我们成功地降低了成本和DHM的大小。成本较低超过20000日元(约240美元在83日元/美元),它大约是×120毫米80毫米55毫米大小×。此外,通过调整记录距离一个全息图,在最合适的距离的横向分辨率17.5μM因此,本文在田野调查的DHM,教育是有用的,等等。此外,我们可以通过使用一个网络摄像头和图像的实时获取图像重建处理单元(GPU)加速[ 6 ]。

本文的结构如下。2部分,介绍了记录一种全息重建方法。3节和4解释硬件配置和使用的软件。5部分评价系统。最后一节讨论了研究结果并提出了进一步的研究。

2。记录和再现方法

本研究的目的是实现价格的降低和缩小DHM。因此,我们采用直列式或作为全息图记录装置,不需要光学零件,如镜片[ 7,8 ]。的直插式DHM的概念图图1所示。因此,没有必要将物光和参考光不像在Mach森德型DHM,从而降低了透镜的成本。接下来,我们解释的重建技术。在分布式水文模型,计算机模拟执行从一个全息图重构。在这里,为了追求实时重建,有必要改变光的传播公式为手持设备和低成本的数字全息显微镜

Figure 1. Conceptual figure of in-line type DHM.

这是很容易控制的计算机。角谱法,菲涅耳衍射,夫琅禾费衍射,等等都是近似技术。这些都是正确的根据条件,如传播距离。为了实现精简DHM,是本研究的目的,有必要缩短

对象和全息图的边之间的距离(电荷耦合器件(CCD)相机)。因此,我们采用角谱方法,这是有效的的对象和全息图的边的距离很短。角谱法如下图所示。

在这里,该算子F [·]和[ ] 1 F?·表明傅里叶逆傅里叶变换,和下标X,Y,Z表示,水平,垂直,深度和组件。(,)和(X,Y)的全息图面和观察面坐标,分别。D是全息图的边观察边之间的距离,和是光的波长。Fx,Fy和Fz的空间频率成分每个轴,和FZ如下。

如果我们计算使用角谱方法,离散傅里叶变换(DFT)计算规模大,在高速的快速傅里叶变换(FFT)。因此,它是可能的执行和记录的全息图重建计算机仿真,如果我们使用GPU 的重建,可实时。

Handheld and low-cost digital holographic microscopy

Figure 2. Composition of the handheld DHM system to create.

3。硬件设计

为了实现低成本和裁员的分布式水文模型,我们建立了硬件如图2。我们使用一个网络摄像头C910摄像头,由Logicool,为CCD相机和被聚焦的图像在网络摄像头的镜头CCD相机由于镜头不是DHM的应用需要。的分辨率此Web摄像头拍摄的1920×1080像素。使用商用网络摄像机,成本可以大幅降低。的ostcxbc1c1s,是一种高功率RGB—

发光二极管(LED)由optosupply,作为光源。这LED的特点,允许一个350毫安的电流是通过它的能力。另一个特征是具有三个波长,470 nm,525 nm,625 nm。这光

LED作为穿过针孔点光源。的直径针孔是5μM(由光洋针孔产品编号是2412)。虽

针孔一般位于物镜焦面,在我们的研究中,该完美的无透镜光学系统的使用而不是降低成本的物镜。作为一个替代,针孔直接连接到LED。

此外,为了调整光源,距离的对象,和一个CCD相机,使用可变长度的支持。实际DHM 系统显示在图3。当记录全息图,图3的实际DHM系统安装在一个阴影的情况下。

4。软件设计

用于记录全息图和全息图的重建软件是必需的。opencv [ 9 ],这是开放源代码库,由英特尔开发和发布,用于记录的全息图。利用opencv,一个网络摄像头控制和编辑一个记录的全息图进行。此外,它是必要的计算从光的衍射的全息图重建。

手持设备和低成本的数字全息显微镜

图3。DHM系统照片。(一)没有阴影的情况下DHM系统。(b)

手持DHM系统。

Table 1. Parts used and costs for the DHM system.

parts costs[yen]

CCD camera 7,092

high power RGB-LED 500

针孔9,800

阴影的情况下1,400

total 18,792

因此,CWO + +库,一种光学计算的开源库,使用[ 10 ]。催化湿式氧化+ +图书馆设有几个命令时使用的光学计算。因此,本研究采用角谱法计算在CPU或GPU计算的一个简单的描述。因此,利用OpenCV和CWO + +库是免费的,使编程简化和改进在计算速度。

5。性能

5.1。成本和尺寸零件的使用成本,如表1所示。如表1所示,它是由不到20000日元(约250美元在80日元/美元)。此外,该大小或等于遮光箱子的尺寸,它是120毫米80毫米55××mm。

Handheld and low-cost digital holographic microscopy

图4。元素5集团4的美国空军测试目标数的全息图

图5。元素5集团4的美国空军的号码图像重建测试目标。

5.2。横向分辨率的功率测量

为了测量创造了这个DHM系统横向分辨率研究中,采用USAF1951测试模式。此外,与短的蓝色光波长作为光源。提高横向分辨率,我们调整位置的CCD摄像机和使用可变长度的对象支持。在这个系统中,横向分辨率是最高时的距离从光源到对象为3毫米,从光源的距离对CCD摄像机为13毫米。在这种情况下,记录全息图所示图4。此外,从图4得到的重建图像显示图5。图4和图5显示,那些已经解决了阵元数54组数。如上所述,本DHM的横向分辨率,功率为17.5μM

6。结论与讨论

在这项研究中,我们降低了成本或小于20000日元(约250在80日元/美元美元),并减少了大小120毫米80毫米55毫米××。这个DHM的横向分辨力为17.5μM。

DHM裁员实地调查是有用的,在原理和使用一个开放的成本降低手持设备和低成本的数字全息显微镜7源库的教育环境,有很大的好处。此外,由于对RGB LED光源用在这项研究中,不同波长可以控制。这是适用于使用两个波数字全息波前复原[ 2 ],两波拉普拉斯重建[ 11 ],或两个波拉普拉斯重建[ 12 ]。在这项研究中,由于采用角谱方法,重建图像可以在相同的分辨率得到的全息图。缩放的角谱方法[ 13 ],可以计算在不同采样率对全息图衍射重建的图像是有用的因为它能够观察这个DHM系统一个详细的重建图像与一个较小的采样率对重建图像。添加这样一个功能,方便今后的学习或提高考虑。

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