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外文资料及中文翻译

外文资料及中文翻译
外文资料及中文翻译

中文译文

从新型葡萄籽IH636中提取的低聚原花青素和盐碱酸内的铬对治疗仓鼠的动脉粥样硬化症有良好的作用

摘要:动脉硬化症是大动脉上的一种疾病,患有此病在内部动脉壁上会长有脂肪扁桃体颗粒,这些脂肪颗粒最终会堵塞血液流通。以确认的导致动脉硬化症的危险因素包括:遗传、饮食习惯、生活方式、抽烟、循环流通着的脂质和胆固醇含量以及慢性血管炎症和流通信号。黄酮类化合物和动脉硬化症之间有一定的关系,主要是由于黄酮类化合物具有抗氧化剂类的性质,并且黄酮类化合物还有抑制体内低浓度脂蛋白的氧化作用。美国人普遍存在着血脂醇过高现象,而这正是导致心脏血管疾病的主要危险因素。从葡萄籽中提取的低聚原花青素已被证实具有广泛的化学保护特性和心脏保护特性以免受氧危害。最近的一项研究表明从IH636葡萄籽中提取的低聚原花青素与NBC一起使用能降低血脂醇过高的人体中的胆固醇及低浓度脂蛋白和被氧化的脂蛋白总含量。本实验通过分别给仓鼠单独喂食葡萄籽中的低聚原花青素及和NBC一起服用,证实了低聚原花青素对仓鼠的动脉硬化症有益处。因为当给仓鼠服用含0.2%的胆固醇、10%的椰子油的高血胆脂醇食物时他们就会长有与血胆脂醇过多的人像似的脂脂膜。仓鼠食用10周椰子油后就会长出泡沫细胞这是动脉硬化症早期的生物标志。与椰子油一起服用,当低聚原花青素的浓度为50mg/kg 、100mg/kg时,仓鼠得动脉硬化症的几率分别降低50%及60%。若低聚原花青素与NBC一起使用时患病几率大约降低32%。每一实验组用7~9只仓鼠。无论是单独使用葡萄籽中的低聚原花青素或是与NBC 一起使用都对胆固醇、甘油三酸酯的含量,不仅如此还对氧化性脂质损害有很好的抑制作用。这一点已通过硫酸反应物的形成所证明。这一数据表明葡萄籽提取物低聚原花青素(GSPE)和烟碱酸内的铬(NBC)可以提供良好的健康因子。这是通过降低泡沫细胞的形成从而大幅度改变动脉硬化症的发生率来实现的。

关键词:GSPE NBC 动脉硬化症仓鼠泡沫细胞胆固醇甘油三酸酯硫酸反应物

引言

氧分压升高是血胆脂醇过高的一个主要标准,这导致了动脉粥样硬化。心脏内氧分压的形成是由于产生了过量的自由基,正是这些自由基引起了脉管组织的损伤。动脉粥样硬化症是一种疾病,此病可使内部动脉壁上产生脂肪条纹,从而堵塞了血液流通,动脉硬化症及其并发症如冠心病、心肌梗塞、心肌梗死和心脏中风这些都是导致死亡的头号杀手。患有动脉硬化症的巨噬细胞和平滑肌肉细胞内中无法控制的胆固醇和积累的脂质将导致泡沫细胞的形成和一系列动脉粥样硬化薄片。通过对一种新型葡萄籽IH636种提取的低聚原花青素进行多次研究后证实了低聚原花青素具有绝好的清除氧自由基和保护心脏的特性。GSPE具有清除生物反应产生的自由基功能,如:超氧化阴离子、羟基自由基和过氧化氢自由基,并且比维生素C 、维生素E和β-胡萝卜素的抗氧化性能力更强更优越。此外,葡萄籽中的低聚原花青素对老鼠体内损伤和心肌局部缺血有独特的保护功能,并且是亚德里亚霉素诱发了老鼠心脏中毒性物质的产生。近期的一项人体研究发现葡萄籽中的低聚聚原花青素和NBC一起作为氧调和铬的盐碱聚合体可以明显的降低人体内的胆固醇、低浓度脂蛋白和被氧化的低浓度脂蛋白的总含量。

本实验的研究就是为了确定葡萄籽中的低聚原花青素是否可以阻止泡沫细胞的形成即是否可能阻止动脉粥样硬化症的形成。仓鼠连续10周食用含0.2%的胆固醇、10%的椰子油的血胆脂醇过高的食物,使这些动物体内产生泡沫细胞,这是早期动脉粥样硬化症的生物标志。葡萄籽中低聚原花青素的保护功能是由其用量所决定的,这是通过分别单独使用花青素及与NBC一起使用使胆固醇和甘油三酸酯的含量有所降低上来确定的。而且在此类仓鼠中也证实了硫酸反应物和泡沫细胞确实减少了。

原料和方法

化学原料

GSPE—作为商业上所公认的苯丙酸诺龙(商品名)和NBC—作为商业上所公认的铬合金巴拉圭茶均来自美国加州一所内部营养食品健康合作机构。从葡萄籽中提取的低聚原花青素是一种天然提取物,含有大约76%的齐聚物花青素和3%的单节显性生物黄酮类化合物,通过对严重的口腔病、皮肤病和初级眼疾病的研究及为期90天的慢性口腔毒性和长期毒性的研究,证实了GSPE是安全的并无任何毒副作用,最近低聚原花青素又通过了一致的安全认定。NBC是一种独特的铬

复合物可以很好的提高人体内的生物功能及铬的生物活性,增强胰岛素的新陈代谢作用。老鼠体内每千克体重大约含有一克铬中静脉内的脂质蛋白,而且在食物中添加盐碱酸铬的浓度达4.6g/kg连续使用20周,并未出现生长缓慢或其他毒副作用。美国环境保护局科技部人类危险性鉴定组织调查了铬的安全性和毒性,并且确定了相关剂量标准为:每天每千克食物中铬含量为1000微克。除非有其他明文规定,所有化合物和试剂均来自斯格码化学所,并且是分析级或最高级。动物和治疗

本实验所用的是雄性、刚断奶的叙利亚婴幼仓鼠来自查尔斯饲养实验室并且连续四周喂食商业的未纯化的啮齿目动物食物。尔后这些动物被分成数组,每组都具有相对均匀的重量。

这些动物饲养在塑料笼子里,每只笼子有3~4只老鼠,笼子内设有木条小床,温度控制在室温(20℃),光照周期光/黑暗为12小时,给他们提供充足的食物和水。总之这些动物的饲养遵循了Scranton 大学的指南协会动物护养和用途委员会的指导方针。

为实验组的动物提供高胆固醇和高脂肪的食物,简要地说就是1000克粉状啮齿目动物食物中加入600毫升水,并且含有0.2%的胆固醇和10%的椰子油,而后融化混合物。除此之外,水中还要加入十个独自的地甜味剂。低花青素食物中大约每千克啮齿目动物食物含有0.88克低聚原花青素,而高花青素食物中大约每千克啮齿目动物食物含有1.76克低聚原花青素。在低花青素食物中每千克原花青素大约加0.88克NBC,每千克啮齿目动物食物加40毫克,半固体混合物就会凝固,从而分裂成综合色物质。

在喂养十周后接着给仓鼠断食12小时,用戊巴比妥将其麻痹,从而造成心脏穿孔。在含有乙二酸四乙胺的微量容器内血液就会聚集,学浆被分离,在90℃下储存一作分析使用。麻痹后的仓鼠喷洒含10%的甲醛磷酸缓冲液从而分离出大动脉已备组织学研究使用。

按Kowalaet al所描述的方法进行解刨和分析。从离心脏1毫米处截取大约4~5毫米长的一段大动脉,进行脱脂、修饰并拥油红O着色。在16x40倍放大镜下观察上层样品以确定泡沫细胞的数量、大小和分布。

泡沫所覆盖的面积用微型放映机放大后可通过电脑数字垫料块进行测定。甘

油三酸酯总量可用斯格码酶分析技术测定出来。胆固醇总量可用斯格码配套元件测定。通过斯格码磷酸盐试剂降低浓度脂蛋白沉淀后,高浓度脂蛋白可用相似的方法测定。

为了确定GSPE在仓鼠体内的作用,使用了一种体内型仓鼠,因为当给仓鼠服用含0.2%的胆固醇、10%的椰子油的高血胆脂醇食物时他们就会长有与血胆脂醇过多的人像似的脂脂膜。仓鼠食用10周椰子油后就会长出泡沫细胞这是动脉硬化症早期的生物标志。在喂食GSPE的动物中可以测定动脉粥样硬化的指数。结果

仓鼠的食物中葡萄籽中低聚原花青素的含量分别为50g/kg和100g/kg, 都连续10周服用含0.2%的胆固醇、10%的椰子油的高血胆脂醇食物,结果得动脉粥样硬化症的几率分别降低了50%和63%。在同样的条件下,若50g/kg的低聚原花青食物加入NBC的病几率就降低32%。

每组动物的食物消耗量和体重并未出现任何异样。实验前仓鼠的平均体重是86±6g,试验结束后四组仓鼠的平均体重和标准偏差控制在:151±26g,低聚原花青素食物:155±9g, 高聚原花青素食物:154±14g,低聚原花青素食物加NBC:148±9g。每只仓鼠平均每天消耗的食物大约为11g。

表一:不同浓度的葡萄籽中低聚原花青素分别单独使用及与盐碱酸中的铬同使用时对胆固醇、三油酸甘酯、血浆油脂氧化前体(TBARS)含量及大动脉上泡沫细胞的百分比,泡沫细胞是体内大动脉的一种标志。

组别胆固醇

(mg/dl)

三油酸甘

(mg/dl)

动脉粥样硬化症

(% 大动脉上泡沫细胞所

占百分比)

TBARS

(μM)

Control (n = 8)592 ±

40.0

898 ±

767

5.93 ± 3.64%

0.285 ±

0.039

GSPE (50 mg/kg) (n = 9)443 ±

49.0

806 ±

433

3.00 ± 0.44%*

0.379 ±

0.070

GSPE (100 mg/kg) (n = 9)457 ±

67.0

593 ±

401*

2.19 ± 0.28%**

0.311 ±

0.157

GSPE (50 mg/kg) + NBC (2.2 mg/kg) (n = 7)346 ±

103*

203 ±

122*

4.01 ± 1.96%

0.066±

0.022***

*p < 0.1; **p < 0.05; ***p < 0.001.

食物中葡萄籽中低聚原花青素的含量分别为50g/kg和100g/kg,连同高脂蛋

白一起服用,总胆固醇含量分别降低了25%和34%,甘油三酸酯分别降低10%和34%。

若低原花青素食物再加上NBC总胆固醇量就降低42%甘油三酸酯降低了77%(如图表一所示)。

无论是否含有低聚原花青素,血浆脂质的前氧化体都未有明显的变化。但是若低聚原花青素(50g/kg)再加上NBC血浆脂质前氧化体就降低了77%(如表一所示)。

讨论

动脉硬化症及其并发症如冠心病、心肌梗塞、心肌梗死和心脏中风被定为发达国家中导致死亡的都号杀手。高血压、糖尿病、抽烟和食用高胆固醇高脂肪的食物都有可能过早地患有动脉粥样硬化症。此外,个人的遗传因素和与众不同的生理特性在这种病理学上也发挥着很重要的作用。患有动脉硬化症的巨噬细胞和平滑肌肉细胞内中无法控制的胆固醇和积累的脂质将导致泡沫细胞的形成和一

系列动脉粥样硬化薄片,这一点众所周知。更奇怪的是高含量的低浓度脂质蛋白与动脉粥样硬化症一起产生。当巨噬细胞流过内皮血管病吞噬氧化脂质时,由于自由基所产生的氧化低浓度脂质蛋白对血管内形成的脂肪条也有很大的影响。葡萄籽中的低聚原花青素可以保护老鼠免收心肌局部缺血剂梗塞的损害,这是通过有规律的改变基因cJUN、JNK1来实现的。

体内氧压增高是血胆酯醇过高的一个主要特征,正是血胆酯醇过高导致了动脉粥样硬化症,氧化活性的升高可以调节血管组织免收伤害。有多种方法可以降低低浓度脂质蛋白胆固醇的含量。降低胆固醇含量的最简单方法就是通过饮食、新型抗氧化物和运动锻炼。一种有利的抗氧化物可以作为治疗动脉粥样硬化症的化学保护剂。以前的体内研究就说明了GSPE对保护由于抽烟所引起的氧化压力和人类处级组织中的死亡细胞有很好的作用。通过体内和体外的研究,证实了GSPE 具有清除生物反应产生的自由基功能,如:超氧化阴离子、羟基自由基和过氧化氢自由基,并且比维生素C 、维生素E和β-胡萝卜素的抗氧化性能力更强更优越。不仅如此,低聚原花青素对保护由于阿霉素所引起的心脏中毒更有效果。

长久以来就已证实缺少铬对患动脉硬化症有决定性的作用。早期研究就表明了铬可以降低胆固醇和三油酸酯的含量。铬的这种作用机制是通过调节葡萄糖和胰岛素的代谢途径并且对心绪不宁也有一定的作用。NBC对降低人类和动物血液中的胆固醇和血脂酯也有很好的效果和安全性。并且它在动物体内有很高的生物

活性,可以自发地防止老鼠由于高糖所产生的高血压及维护糖的良好浓度,降低肝肾脏的氧化前体。在对一项人体的临床研究中,表明NBC可以有效地降低免疫学清的适度含量,平均降低了14%,并且使男性运动员体内的胆固醇和高脂质蛋白的比提高了7%。

GSPE和NBC的作用分通过单独和一起在布朗.挪威菲斯格棕色老鼠身上进行的实验得到了验证。这些研究证实了低聚原花青素和NBC单独服用或一起服用都可以明显地降低心脏收缩血压,影响葡萄籽—胰岛素而且凝乳血管紧素缩系统在血压正常和高血压的鼠内均没有导致任何明显的毒性作用。

对随意抽取的40个胆血脂醇过高的人进行临床研究。这些人或服用安慰剂或服用100mgGSPE和200vgNBC,每天服用两次,连续服用2个月。GSPE和NBC一起服用使胆固醇总量和低浓度脂质蛋白胆固醇分别降低16.5%和20%,单独服用GSPE及GSPE和NBC共同服用LDL含量分别降低了30.7%和44.0%,而安慰剂和NBC单独服用分别降低了17.3%和10.4%。

治疗动脉粥样硬化症最好的药物如:利普妥、辛戈他等虽然都能很好地降低胆固醇含量和欢心脏病的几率,但是则写药物都有很大的副作用如造成肌肉酸痛和人失忆,这也是早衰的原因。肌肉酸痛是由于骨骼肌肉细胞大量死亡,严重时还会导致人或其他生命的死亡。也有些药物可使肝细胞和肾脏受损,而GSPE对肝细胞和肾脏有很好的保护功能,且无任何副作用。

最近一项研究发现GSPE和NBG之间并无协同作用,这也许与我们所选的实验动物类型有关。还发现无论是葡萄汁还是高纯度红酒都能有效地降低仓鼠患动脉粥样硬化症的几率。并且红酒中对心脏有益的因子是从GSPE中获得的。这些数据并同以前的研究都说明了GSPE和NBC保护心脏的机制是不同的。总之,GSPE和NBC 都是改良人体和动物动脉粥样硬化症的新型治疗工具。

英文论文及中文翻译

International Journal of Minerals, Metallurgy and Materials Volume 17, Number 4, August 2010, Page 500 DOI: 10.1007/s12613-010-0348-y Corresponding author: Zhuan Li E-mail: li_zhuan@https://www.doczj.com/doc/316284940.html, ? University of Science and Technology Beijing and Springer-Verlag Berlin Heidelberg 2010 Preparation and properties of C/C-SiC brake composites fabricated by warm compacted-in situ reaction Zhuan Li, Peng Xiao, and Xiang Xiong State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China (Received: 12 August 2009; revised: 28 August 2009; accepted: 2 September 2009) Abstract: Carbon fibre reinforced carbon and silicon carbide dual matrix composites (C/C-SiC) were fabricated by the warm compacted-in situ reaction. The microstructure, mechanical properties, tribological properties, and wear mechanism of C/C-SiC composites at different brake speeds were investigated. The results indicate that the composites are composed of 58wt% C, 37wt% SiC, and 5wt% Si. The density and open porosity are 2.0 g·cm–3 and 10%, respectively. The C/C-SiC brake composites exhibit good mechanical properties. The flexural strength can reach up to 160 MPa, and the impact strength can reach 2.5 kJ·m–2. The C/C-SiC brake composites show excellent tribological performances. The friction coefficient is between 0.57 and 0.67 at the brake speeds from 8 to 24 m·s?1. The brake is stable, and the wear rate is less than 2.02×10?6 cm3·J?1. These results show that the C/C-SiC brake composites are the promising candidates for advanced brake and clutch systems. Keywords: C/C-SiC; ceramic matrix composites; tribological properties; microstructure [This work was financially supported by the National High-Tech Research and Development Program of China (No.2006AA03Z560) and the Graduate Degree Thesis Innovation Foundation of Central South University (No.2008yb019).] 温压-原位反应法制备C / C-SiC刹车复合材料的工艺和性能 李专,肖鹏,熊翔 粉末冶金国家重点实验室,中南大学,湖南长沙410083,中国(收稿日期:2009年8月12日修订:2009年8月28日;接受日期:2009年9月2日) 摘要:采用温压?原位反应法制备炭纤维增强炭和碳化硅双基体(C/C-SiC)复合材

关于力的外文文献翻译、中英文翻译、外文翻译

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第1章英文阅读材料翻译 (1) 第2章中文摘要翻译英文 (3) 第3章中文简历和英文简历 (4) 第4章课程学习体会和建议 (6) 参考文献 (7)

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